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Single-use bioreactor - Wikipedia

Single-use bioreactor - Wikipedia

The reactor is operated in a perfusion mode with the shaft rotation speed maintained at a constant RPM. ... Sikavitsas VI, Mikos AG. Design of a flow perfusion bioreactor system for bone tissue-engineering applications. Tissue Eng. 2003; 9: ... Singh V. Disposable bioreactor for cell culture using wave-induced agitation. model system, the thesis focuses on High Cell Density Culture (HCDC) perfusion process development and covers mainly two parts. The first part is HCDC perfusion system development with our industrial collaborators. The second part addresses the HCDC perfusion process development and highlights

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GE Wave Bioreactor 20/50 EH - The Lab World Group

GE Wave Bioreactor 20/50 EH - The Lab World Group

The results of this study demonstrate that a stirred-tank bioreactor system could be used for more efficacious T-cell expansion and reduced cost of goods with shortened culture periods. References 1 Turtle CJ, et al. CD19 CAR-T Cells of Defined CD4 :CD8 Composition in Adult B Cell ALL Patients. NSO, S2 and SF9 have been cultivated successfully in WAVE Bioreactor systems, demonstrating the flexibility of this device (1-5). In a WAVE Bioreactor system (Fig 1) the cells are grown in a pre-sterilized, inflated cultivation chamber, a Cellbag bioreactor. The culture is agitated by rocking the bag attached to the rocker tray.

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Biostat RM and Flexsafe RM for Seed Production and Small ...

Biostat RM and Flexsafe RM for Seed Production and Small ...

The WAVE Bioreactor™ system from GE Healthcare is a functionally closed system and can be used for various cell culture applications. The video illustrates the workflow for a perfusion culture working with the WAVE Bioreactor 2/10 system. The WAVE Bioreactor system produces viruses under complete containment without the need for biosafety cabinet. Insect cell / Baculovirus. The high oxygen supply capability of the WAVE Bioreactor system makes it ideal for insect cell culture.Cell densities over 9x106 are routinely achieved.

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WAVE MIXER - AIE

WAVE MIXER - AIE

The ergonomic rocker design makes handling convenient and easy. The ability to handle the bioreactor in a tilt position facilitates sampling and harvest. The wide top opening facilitates for the user to interact with the culture without removing the lid. End-user license agreement for ReadyToProcess WAVE 25 system (A and B) Block diagram and photo of the Bioreactor System (ultrasound device not shown). (A) The bioreactor has an interior circuit, where media flows through the graft creating the proper shear stress and pressure conditions as well as maintaining the cells, and an outer circuit providing an interstitial environment with low flow for perfusion.

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Bioreactors for animal cell suspension culture

Bioreactors for animal cell suspension culture

They can be applied to specific cells and processes, such as the bubble-free, ventilated travelling wave bioreactor for cell culture [18, 19]. If orbital-shaking is combined with in situ substrate release technologies [ 20 , 21 ] or micromechanical feeding [ 22 ], the disadvantages of a more difficult mechanical feeding of nutrient solution to those systems are compensated. bioreactors. The Wave Bioreactor system is extremely easy to operate and inoculum scale-up and infection can be done inside the bioreactor, reducing the need for transfers. Liters SYSTEM500/1000 100-500 PROVEN FOR: Insect Cell/Baculovirus Plant Culture CHO HELA NS0 Microbial cGMP Production Microcarriers Perfusion Culture Monoclonal Antibodies ...

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How scalable and suitable are single-use bioreactors ...

How scalable and suitable are single-use bioreactors ...

 · The WAVE Bioreactor™ system from Cytiva is a functionally closed system and can be used for various cell culture applications. The video illustrates the workflow for a perfusion culture working ... WAVE 25 rocking bioreactor system volumes. The system can automatically determine suitable parameters for a run, a feature that facilitates fast start‑up and enables accurate and stable control. Easy to use ReadyToProcess WAVE 25 system is designed for ease of use. Cellbag bioreactor attachment is straightforward and system

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Demonstrating Scalable T-Cell Expansion in Stirred-Tank ...

Demonstrating Scalable T-Cell Expansion in Stirred-Tank ...

PDF | On , T.C. Zhou and others published Cell culture, bioreactors, commercial production. Encyclopedia of Industrial Biotechnology | Find, read and cite all the research you need on ... BIOSTAT® RM 20 | 50 basic is a perfectly sized, single-use, wave-mixed benchtop bioreactor for stand-alone use. It features an exchangeable bag holder to fit bags with a total volume of 1 – 50 L. The BIOSTAT® RM Basic rocking platform with an integrated local controller, Air | CO2 mixing module and optional load cells is the optimal choice for straight-forward applications, such as seed ...

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A flow perfusion bioreactor with controlled mechanical ...

A flow perfusion bioreactor with controlled mechanical ...

 · Regarding the cell retention in perfusion systems, a large variety of examples can be found for the production of recombinant proteins (Pollock et al. 2013).In general, filtration-based systems (i.e., internal and external spin filter, ATF and TFF), gravity settlers, and acoustic filters have been extensively used in the industry as well as in academia (Clincke et al. 2013; Kompala and Ozturk ... Wave Bioreactor 15. Wave-induced Agitation 8/14/2015 15 16. ADVANTAGE OF WAVE BIOREACTOR Disposable bioreactor chamber-no cross-contamination, cleaning, sterilization or other validation headaches. Seed preparation-Seed culture can be prepared in the final system itself, i.E. Batch can be started with 100ml and can go to 2000ml.

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ReadyToProcess™ WAVE 25 rocking bioreactor system

ReadyToProcess™ WAVE 25 rocking bioreactor system

After five days of stable perfusion, the culture was ended. Fig 4. Cell growth and viability for perfusion culture using TFF as cell retention method. Fig 5. CSPR, product titer, and vessel volumes per day (VVD) for perfusion culture using TFF as cell retention method. Fig 6. Perfusion setup with XDR-10 bioreactor system, using ATF as cell Perfusion is not exactly new for cell culture (Croughan et al., 2015).From the early 90s to today, different commercial molecules such as recombinant follicle stimulating hormone (Gonal-f©), interferon beta-1a (Rebif©) and factor-VIII (Kogenate-FS©) were launched using perfusion processes (Pollock et al., 2013).Most of these molecules are labile and their indication range is expressed in ...

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Perfusion bioreactor with TFF cell retention | Cytiva ...

Perfusion bioreactor with TFF cell retention | Cytiva ...

Labfors 5 is a leading bench-top bioreactor, providing the user with an easy to handle, flexible and upgradeable system for culture volumes from 0.5 L up to 10 L. The Labfors 5 is always application optimized for your bioprocesses. Different cultivation strategies such as culture in batch, fed-batch and continuous modes are possible. The perfusion bioreactor, in which the culture medium is directed to flow through the pores of the scaffold, is superior compared to other nonperfusion or static bioreactors by enhanc-

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In Vitro Technologies :: Bioreactors

In Vitro Technologies :: Bioreactors

A single-use bioreactor or disposable bioreactor is a bioreactor with a disposable bag instead of a culture vessel. Typically, this refers to a bioreactor in which the lining in contact with the cell culture will be plastic, and this lining is encased within a more permanent structure (typically, either a rocker or a cuboid or cylindrical steel support). The medium and gas entering the bioreactor via the perfusion system provide metabolic support for the high-density cell growth, and thus 2-D large-scale culture of adherent cells is feasible. This bioreactor provides a sufficient surface for the large-scale culture of adherent cells, and the compact design between plates abolishes the gas phase between plates.

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Bioreactor | Cambridge Scientific

Bioreactor | Cambridge Scientific

This study demonstrates perfusion culture of an antibody-producing CHO cell line, with tangential flow filtration (TFF) as cell retention method. The setup includes a single-use stirred-tank bioreactor system, Xcellerex XDR-10, and a single-use hollow fiber filter cartridge as cell retention device. The GE WAVE Bioreactor 20/50EH is a flexible system built for use in Research and Development and the pharmaceutical production arena. Its modular system consists of a base unit and various integral options suitable for the culture of multiple cell lines. The benchtop design makes for easy access and convenient control.

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Perfusion culture made easy with WAVE Bioreactor™ 2/10 …

Perfusion culture made easy with WAVE Bioreactor™ 2/10 …

Unique bi-axial agitation improves cell culture performance. The Allegro XRS 25 bioreactor system is a single-use bioreactor system with unique agitation and control properties designed for the cultivation of mammalian cells in suspension culture under controlled conditions. The present invention relates to a bioreactor and a method for operating a bioreactor which comprises a chamber(100) capable of receiving a liquid media, and a filter assembly (200) comprising a perfusion filter(210; 212; 214). The filter assembly (200) is disposed in the chamber(100) and the filter assembly (200) is free to move within the chamber(100).

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